简介

酵母生物技术研究组长期开展酵母菌代谢与合成生物学研究。“因菌制宜”地建立了解脂耶氏酵母、红法夫酵母、酿酒酵母、毕赤酵母研究平台，解析底盘的遗传与代谢调控机理，开发基因编辑、亚细胞合成、定向进化等技术，发展高产菌种与发酵工艺，聚焦酵母菌合成油脂和蛋白实现了多项技术转让。在PNAS、Science Advances等期刊发表SCI论文30余篇；申请PCT和发明专利22项，已获中国、美国、日本和欧洲授权12项。承担国家重点研发计划项目和省部级项目多项。

 研究课题

1) 国家重点研发计划项目，发酵法生产功能性营养化学品关键技术，2022YFC2106200，2023.2-2027.1，2412万元

2) 国家重点研发计划项目，秸秆类生物质生产生物柴油与药物中间体技术研发，2022YFE0207100，2022.11-2025.10，120万元/600万元

3) 中国科学院先导C项目，典型工业菌种的构建与应用XDC0110000—饲用蛋白高效表达底盘细胞创制，2023.10-2026.9，90万元

4) 山东省重点研发计划，酵母单细胞蛋白与高附加值饲料添加剂联产关键技术及产品，2022CXGC020712，2022.10-2025.12，40万元/300万元

5) 山东能源研究院科研创新基金，推动产油酵母广泛应用的关键技术及原理，SEI I202136，2021.06-2023.05，300万元

6) 国家自然科学基金面上项目，红发夫酵母油脂积累对虾青素合成的正负调控及机理研究，31670054，2017.01-2020.12，62万元

 研究平台

      分子生物学平台、代谢工程与合成生物学平台、定向进化平台、自动化高通量筛选平台、发酵平台。

  主要成果

1. Ruilin Huang, Jiurong Wang, Ruirui Ding, Yu Liu, Juanjuan Su, Zhihang Chen, Yong Fan, Fuli Li, Linqi Wang, Xiao Liu and Shi’an Wang*. A conserved E3 ubiquitin ligase rewires carotenoid, sterol, and lipid metabolism in Xanthophyllomyces dendrorhous. PNAS, 2026, 123(17):e2530496123

2. Qian Ling^# , Bozhao Li^# , Yuhua Feng, Jing Yang*, Shi’an Wang*, Suping Li*, Cheng Zhang*. Conformation Programmed DNA Computing. Science Advances, 2026, 12(8):eaec8383.

3. Zhihang Chen^#, Jiajun Deng^#, Peijie Han^#, Jiabao Li, Dong Li*, and Shi’an Wang*. Mass production of microbial protein using low-value materials. The Innovation Life, 2026, 4:100200.

4. Juanjuan Su, Shiyou Xing, Wei Qi, Shi’an Wang*. Scaling sustainable aviation fuel from microbial oils: challenges and opportunities. 10 Breakthrough Ideas in Energy for The Next Ten Years (6th Anniversary edition), 2025, 25-33.

5. Shi’an Wang*, Juanjuan Su. Organisms for biofuel production. 10 Breakthrough Ideas in Energy for The Next Ten Years (5th Anniversary edition), 2024, 18-26.

6. Ruirui Ding, Ruilin Huang, Hang Su, Jiawen Li, Fuli Li, Shi’an Wang*. Screening of astaxanthin-overproducing Xanthophyllomyces dendrorhous strains via iterative ARTP mutagenesis and cell sorting by flow cytometry. J Appl Microbiol, 2024, 135(2):lxae020.

7. Hang Su^#, Penghui Shi^#, Zhaoshuang Shen^#, Huimin Meng, Ziyue Meng, Xingfeng Han, Yanna Chen, Weiming Fan, Yun Fa, Chunyu Yang, Fuli Li^*, Shi’an Wang^*. High-level production of nervonic acid in the oleaginous yeast Yarrowia lipolytica by systematic metabolic engineering. Communications Biology, 2023, 6:1125.

8. Qingqing Xu^#, Qingyun Tang^#, Yang Xu, Junjun Wu, Xiangzhao Mao, Fuli Li, Shi’an Wang, Yonghua Wang. Biotechnology in Future Food Lipids: Opportunities and Challenges. Annual Review of Food Science and Technology, 2023, 14:225-246.

9. Ruilin Huang^#, Ruirui Ding^#, Yu Liu, Fuli Li, Zhaohui Zhang^*, Shi’an Wang^*. GATA transcription factor WC2 regulates the biosynthesis of astaxanthin in yeast Xanthophyllomyces dendrorhous. Microbial Biotechnology, 2022, 15(10):2578-2593.

10. Jixuan Hong^#, Ziyue Meng^#, Zixi Zhang^#, Hang Su, Yuxuan Fan, Ruilin Huang, Ruirui Ding, Ning Zhang, Fuli Li*, Shi’an Wang*. Comprehensive analysis of CRISPR-Cas9 editing outcomes in yeast Xanthophyllomyces dendrorhous. The CRISPR Journal, 2022, 5(4):558-570.