WANG Shi'an-一碳生物技术研究中心

Yeast Biotechnology Group

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Yeast Biotechnology Group

日期：2024-01-26 阅读：

Introduction

The yeast biotechnology group has been conducting long-term studies on yeast metabolism and synthetic biology. Focusing on the synthesis of oils and proteins in yeast, we have established research platforms for Yarrowia lipolytica, Xanthophyllomyces dendrorhous, Saccharomyces cerevisiae, and Pichia pastoris, taking a tailored approach for each strain. We have deciphered the genetic and metabolic regulatory mechanisms of these strains, developed techniques such as gene editing, subcellular synthesis, directed evolution, and high-throughput screening, and achieved the transformation and collaboration of multiple technologies. Over thirty papers have been published in journals such as Communications Biology, CRISPR Journal, Bioresource Technology, Biotechnology for Biofuels, Carbohydrate Polymers, and AEM. We have filed two PCT patents (one of which has been granted in the United States, Europe, and Japan) and twelve invention patents, of which eight have been granted. We have led multiple projects including the National Key Research and Development Program and the National Natural Science Foundation.

Research Project

(1) National Key R&D Program Project - Key Technologies for the Fermentation Production of Functional Nutritional Chemicals, 2022YFC2106200, 2023.2-2027.1, 24.12 million RMB.

(2) National Key R&D Program Project - Research and Development of Biomass-based Production Technologies of Biodiesel and Pharmaceutical Intermediates from Straw Biomass, 2022YFE0207100, 2022.11-2025.10, 1.2 million/6 million RMB.

(3) Shandong Province Key R&D Program - Key Technologies and Products for Co-production of Single-cell Protein from Yeast and High-value Feed Additives, 2022CXGC020712, 2022.10-2025.12, 0.4 million/3 million RMB.

(4) Shandong Energy Research Institute (SEI) Research and Innovation Fund - Key Technologies and Principles for Promoting the Wide Application of Oil-producing Yeast, SEI I202136, 2021.06-2023.05, 3 million RMB.

(5) National Natural Science Foundation of China - Regulation and Mechanism of Positive and Negative Control of Astaxanthin Synthesis in Rhodotorula Yeast, 31670054, 2017.01-2020.12, 0.62 million RMB.

(6) National Natural Science Foundation of China - Product-specific Mechanism of Beta-carotene Desaturase in Rhodotorula Yeast, 32202223, 2023.01-2025.12, 0.3 million RMB.

Research platform

Molecular Biology Platform, Metabolic Engineering and Synthetic Biology Platform, Directed Evolution Platform, Automated High-throughput Screening Platform, Fermentation Platform, etc.

Publications

(1) Hang Su^#, Penghui Shi^#, Zhaoshuang Shen^#, Huimin Meng, Ziyue Meng, Xingfeng Han, Yanna Chen, Weiming Fan, Yun Fa, Chunyu Yang, Fuli Li*, and Shi’an Wang*. High-level production of nervonic acid in the oleaginous yeast Yarrowia lipolytica by systematic metabolic engineering. Communication biology (Accepted, 2023)

(2) Ruilin Huang^#, Ruirui Ding^#, Yu Liu, Fuli Li, Zhaohui Zhang* and Shi’an Wang*. GATA transcription factor WC2 regulates the biosynthesis of astaxanthin in yeast Xanthophyllomyces dendrorhous. Microbial Biotechnology, 2022, 15:2578-2593.

(3) Jixuan Hong^#, Ziyue Meng^#, Zixi Zhang^#, Hang Su, Yuxuan Fan, Ruilin Huang, Ruirui Ding, Ning Zhang, Fuli Li*, and Shi’an Wang*. Comprehensive analysis of CRISPR-Cas9 editing outcomes in yeast Xanthophyllomyces dendrorhous. The CRISPR Journal, 2022, 5(4):558-570.

(4) Zhang N, Li JX, Li FL, Wang SA*. Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector. Appl Microbiol Biotech, 2019, 103:963–971.

(5) Wang D, Li FL*, Wang SA*. Engineering a natural Saccharomyces cerevisiae strain for ethanol production from inulin by consolidated bioprocessing. Biotechnology for Biofuels, 2016, 9:96.